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pu6sgrosa26  (Addgene inc)


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    Structured Review

    Addgene inc pu6sgrosa26
    Pu6sgrosa26, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pu6sgrosa26/pU6-sgRosa26-1_CBh-Cas9-T2A-BFP-P2A-Ad4E1B+(Plasmid+%2364219)/pm40315428-437-70-71
    Average 91 stars, based on 4 article reviews
    pu6sgrosa26 - by Bioz Stars, 2026-09
    91/100 stars

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    Construct:

    Article Title: Engineered Feedback Employing Natural Hypoxia-Responsive Factors Enhances Synthetic Hypoxia Biosensors.
    Article Snippet: .. ■ ACKNOWLEDGMENTS The SV40_min promoter construct was a gift from Yvonne Chen.31 pcDNA3 mHIF-1α MYC (P402A/P577A/N813A) (Addgene #44028) was a gift from Celeste Simon.42 Lenti dCAS-VP64_Blast was a gift from Feng Zhang (Addgene #61425).53 PhiC31-Neo-ins-5xTetO-pEF-H2B-Citrin-ins was a gift from Michael Elowitz (Addgene #78099).54 pDsRed2N1 was a gift from David Schaffer (University of California, Berkeley). pEBFP2-Nuc was a gift from Robert Campbell (Addgene #14893).55 pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2AAd4E4orf6 (Addgene #64220; referred to as pPD782), pU6sgRosa26-1_CBh-Cas9-T2A-BFP-P2A-Ad4E1B (Addgene #64219; referred to as pPD720), and pR26 CAG/GFP Asc (Addgene #74285) were gifts from Ralf Kuehn.63,64 The pPart series vectors, pTU series vectors, pLink series vectors, destination vectors, and HEK293FT-LP cell line were gifts from Ron Weiss.37 Supporting Figure 9B was created in BioRender by P .S .D . (2024) (BioRender . com/ m62t381m62t381). ..

    Sequencing:

    Article Title: MicroRNA-7a2 Regulates Prolactin in Developing Lactotrophs and Prolactinoma Cells.
    Article Snippet: .. The genomic sequence of pre-miR-7a2 ± 250 bp was amplified and cloned into the AscI site of the pR26 CAG/GFP Dest plasmid (gift from Ralf Kuehn; Addgene plasmid #74281). sgRosa26-1 was amplified and in vitro transcribed from the pU6sgRosa26-1_CBh-Cas9-T2A-BFP plasmid (gift from Ralf Kuehn; Addgene plasmid #64216). .. The targeting vector, sgRosa26-1, and Cas9 nuclease (NEB) were microinjected into the pronucleus of C57BL/6N zygotes.

    Amplification:

    Article Title: MicroRNA-7a2 Regulates Prolactin in Developing Lactotrophs and Prolactinoma Cells.
    Article Snippet: .. The genomic sequence of pre-miR-7a2 ± 250 bp was amplified and cloned into the AscI site of the pR26 CAG/GFP Dest plasmid (gift from Ralf Kuehn; Addgene plasmid #74281). sgRosa26-1 was amplified and in vitro transcribed from the pU6sgRosa26-1_CBh-Cas9-T2A-BFP plasmid (gift from Ralf Kuehn; Addgene plasmid #64216). .. The targeting vector, sgRosa26-1, and Cas9 nuclease (NEB) were microinjected into the pronucleus of C57BL/6N zygotes.

    Clone Assay:

    Article Title: MicroRNA-7a2 Regulates Prolactin in Developing Lactotrophs and Prolactinoma Cells.
    Article Snippet: .. The genomic sequence of pre-miR-7a2 ± 250 bp was amplified and cloned into the AscI site of the pR26 CAG/GFP Dest plasmid (gift from Ralf Kuehn; Addgene plasmid #74281). sgRosa26-1 was amplified and in vitro transcribed from the pU6sgRosa26-1_CBh-Cas9-T2A-BFP plasmid (gift from Ralf Kuehn; Addgene plasmid #64216). .. The targeting vector, sgRosa26-1, and Cas9 nuclease (NEB) were microinjected into the pronucleus of C57BL/6N zygotes.

    Plasmid Preparation:

    Article Title: MicroRNA-7a2 Regulates Prolactin in Developing Lactotrophs and Prolactinoma Cells.
    Article Snippet: .. The genomic sequence of pre-miR-7a2 ± 250 bp was amplified and cloned into the AscI site of the pR26 CAG/GFP Dest plasmid (gift from Ralf Kuehn; Addgene plasmid #74281). sgRosa26-1 was amplified and in vitro transcribed from the pU6sgRosa26-1_CBh-Cas9-T2A-BFP plasmid (gift from Ralf Kuehn; Addgene plasmid #64216). .. The targeting vector, sgRosa26-1, and Cas9 nuclease (NEB) were microinjected into the pronucleus of C57BL/6N zygotes.

    In Vitro:

    Article Title: MicroRNA-7a2 Regulates Prolactin in Developing Lactotrophs and Prolactinoma Cells.
    Article Snippet: .. The genomic sequence of pre-miR-7a2 ± 250 bp was amplified and cloned into the AscI site of the pR26 CAG/GFP Dest plasmid (gift from Ralf Kuehn; Addgene plasmid #74281). sgRosa26-1 was amplified and in vitro transcribed from the pU6sgRosa26-1_CBh-Cas9-T2A-BFP plasmid (gift from Ralf Kuehn; Addgene plasmid #64216). .. The targeting vector, sgRosa26-1, and Cas9 nuclease (NEB) were microinjected into the pronucleus of C57BL/6N zygotes.



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    https://www.bioz.com/product/pu6sgrosa26/pU6-sgRosa26-1_CBh-Cas9-T2A-BFP-P2A-Ad4E1B+(Plasmid+%2364219)/pm40315428-437-70-71
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